Catalog Number: 05169291190
IRON2: ACN 8661
In vitro test for the quantitative determination of iron in human serum and plasma on Roche/Hitachi cobas c systems.
|Shelf life at 2-8 °C:||See expiration date on cobas c pack label.|
|On-board in use and refrigerated on the analyzer:||2 weeks|
|On-board on the Reagent Manager:||24 hours|
|When removing the cobas c pack from the instrument during use, please immediately store at 2-8 °C.Do not shake the cobas c pack to avoid foaming.Store protected from light.|
Specimen collection and preparation
|cobas c 701/702 test definition|
|Assay type||2-Point End|
|Reaction time / Assay points||10 / 18-30|
|Wavelength (sub/main)||700/570 nm|
|Units||µmol/L (µg/dL, mg/L)|
|Reagent pipetting||Diluent (H2O)|
|Sample volumes||Sample||Sample dilution|
Traceability: This method has been standardized against a primary reference material (SRM 937).
|Calibrators||S1: H2OS2: C.f.a.s.|
|Calibration frequency||2-point calibration▪after cobas c pack change▪as required following quality control procedures|
0.90-179 µmol/L (5.00-1000 µg/dL, 0.05-10.0 mg/L)
Lower detection limit of the test
Representative performance data on the analyzers are given below. Results obtained in individual laboratories may differ.
Precision was determined using human samples and controls in an internal protocol with repeatability (n = 21) and intermediate precision (3 aliquots per run, 1 run per day, 21 days). The following results were obtained:
Iron values for human serum and plasma samples obtained on a Roche/Hitachi cobas c 701 analyzer (y) were compared with those determined using the corresponding reagent on a Roche/Hitachi cobas c 501 analyzer (x).