LightCycler® 2.0 Instrument

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Overview

 

Simple. Reliable. Flexible.

  • Fast and sensitive real-time PCR and RT-PCR
  • Reproducible and reliable kinetic quantification of DNA, cDNA, or RNA
  • Easy PCR product identification and characterization by melting point analysis
  • Highly specific mutation analysis and SNP (Single Nucleotide Polymorphism) genotyping

 

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View technical specifications

The technology used for the LightCycler is licensed from Idaho Technology, Inc.

lightcycler-2-open-4577-312X440.jpg

 

Features

Fast, reproducible results.
Real-time, online PCR.

Proven LightCycler® instrument technology with enhanced flexibility provides a simple solution for your Real-Time molecular diagnostics needs whether you are adding your lab’s first or fiftieth assay.

Simple.

Software and platform design simplifies instrument implementation as well as assay expansion in your lab.

  • Enhanced capabilities with established methodologies
  • Easy-to-use software
  • Closed tube operation
 

Flexible.

Many options and capabilities provide you with flexibility in routine operation or in the creation of user-defined profiles.

  • Multiple reaction volume options
  • Six detection channels
  • Suitable for clinical and research applications
  • Can run from 1-30 samples
 

Reliable.

Proven reliability allows you to focus on and meet the diagnostic needs of your business..

  • Proven instrument and application history
  • Clinical reputation
  • Data integrity
  • Single vendor solution - Instrument, software, and all consumables designed and optimized to work together and distributed and supported solely by Roche.

Hardware

Multiple reaction volumes Provides flexibility in the development of user-defined applications by utilizing either the 100 uL or 20 uL capillaries.
Six fluorescence detection channels Allows the user flexibility in the selection of detection chemistries that may be utilized in the development of user-defined applications.
Fan design Heating and cooling efficiency allowing for the thermal-cycling of higher reaction volumes without compromising turn around time

Software

Workflow

Gives you the ability through easy navigation to automate your analysis for routine testing or to customize the settings as needed. Creates experimental protocols quickly by utilizing user-defined templates and macros.

Analysis module

Quantification of nucleic acid concentration is possible without performing amplification.

Performs qualitative detection, absolute and relative quantification, along with melting curve analysis for streamlined data evaluation.

Integrated data and user management

Protects user-defined protocols to simplify use for everyone in the lab, resulting in improved data integrity and reproducibility.

Tracks user actions with available audit trail.

Probe design software (optional)

Automatically defines the optimal primers and probes for various Real-Time PCR applications.

Automated analysis and scoring of sequences according to the design parameters.

Import sequence data and select design parameters for your specific applications.

Specifications

Technical Specifications

Dimensions 28 x 38.5 x 50.5 cm +/- 0.5 cm tolerance (W x H x D)
Weight Approximately 22 kg
Power supply 115/230 V; 8A; 50-60 Hz
Power consumption Max. 800 VA
Noise level < 65 dBA
Heat emission 2900 kJ/h (max.) 2100 kJ/h (average value during operation)
Protection class I
Temperatures allowed during transportation/storage/packaging -25°C to +60°C; Relative humidity: 10% to 95% (no condensation)
Temperatures allowed during transportation  +18°C to +30°C
Relative humidity 10% to 95% (no condensation)
Altitude/pressure 0 to 2000 m above sea level 850 – 1050 hP
Safety

Complies with safety standards IEC 61010-1 and IEC 61010-2-101, level of pollution 2, overvoltage category II, CAN/CSA-C22.2 No. 1010.1-92 as well as UL 61010A-1.

UL_logo.bmpComplies with safety standards IEC 61010-1 and IEC 61010-2-101, level of pollution 2, overvoltage category II, CAN/CSA-C22.2 No. 1010.1-92 as well as UL 61010A-1.

The safety mark has been issued by Underwriters Laboratories, Inc. (UL) for Canada and the US.

Excitation

Type high brightness LED
Wavelength (peak) 470 nm +/- 10 nm
Wattage at capillary position in the range of 450nm - 500nm > 0.6 mW 
Filter Interference Filter: Bandpass 470 nm, HBW 40 nm

Filter

Detector channel 1 Interference filter: bandpass 530 nm, HBW 20 nm
Detector channel 2 Interference filter: bandpass 555 nm, HBW 20 nm
Detector channel 3 Interference filter: bandpass 610 nm, HBW 20 nm
Detector channel 4 Interference filter: bandpass 640 nm, HBW 20 nm
Detector channel 5 Interference filter: bandpass 670 nm, HBW 20 nm
Detector channel 6 Interference filter: bandpass 710 nm, HBW 20 nm

Temperature kinetics for PCR

Temperature range 40°C to 98°C 
Accuracy of “mean +/-0.4°C capillary temperature at thermal equilibrium”* +/-0.4°C
Accuracy of “displayed temperature” with respect to capillary temperature at thermal equilibrium* +/-0.3°C (at 50°C and at 95°C)

Capillary heating rates

Heating rate 40°C to 95°C (non-linear) 20μl: < 15 sec, 100μl: < 27.5 sec
Heating rate 50°C to 72°C (non-linear) 20μl: < 8 sec, 100μl: < 11 sec
Heating rate 72°C to 95°C (non-linear) 20μl: < 8 sec, 100μl: < 11 sec

Capillary cooling rates

Heating rate 95°C to 50°C (non-linear) 20μl: < 15 sec, 100μl: < 24 sec
Consumables & Accessories

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QC/Calibration

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